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Synthetic Promoter-Based Azide Biosensor & Protein Induction System
(A) Escherichia coli BW25113-Wt cells with pCyn-v2-GFP plasmid were induced with varying azide concentrations and GFP fluorescence of cell lysate was measured over 24 hours. (B) E. coli BL21 contaning control pEC-GFP plasmid was induced with IPTG (isopropyl β-d-1-thiogalactopyranoside). (C). BW25113-Wt cells with pCyn-v2-GFP plasmid was incubated...
Published: 7/28/2022
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Inventor(s): Shishir Pratap Singh Chundawat, Chandra Bandi, Kyle Skalenko
Keywords(s):
Category(s): Technology Classifications > Physical Sciences & Engineering, Technology Classifications > Healthcare & Life Sciences, Technology Classifications > Food, Nutrition and Nutraceuticals
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High-Throughput Screening of Glycosynthases
Invention Summary:The ability to synthesize specific glycosidic linkages provides a way to produce designer glycans. Glycosytransferases that synthesize oligosaccharides are expressed poorly, have narrow substrate specificity and use expensive nucleotide sugars, limiting their utility in scaling-up glycan synthesis. Glycosynthases (GSs) offer an alternative...
Published: 12/8/2021
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Inventor(s): Shishir Pratap Singh Chundawat, Ayushi Agrawal, Chandra Bandi
Keywords(s):
Category(s): Technology Classifications > Physical Sciences & Engineering, Technology Classifications > Healthcare & Life Sciences, Technology Classifications > Food, Nutrition and Nutraceuticals
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Engineered GH for Improved Glycan Polymer Synthesis
Invention Summary:
Oligosaccharides and polysaccharides (or glycans) are complex macromolecules in living systems that serve critical structural and functional roles. In vitro synthesis of these complex biomolecules is very challenging. Glycosytransferases are naturally available enzymes that catalyze the synthesis of glycans, but have poor expression...
Published: 7/28/2022
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Inventor(s): Shishir Pratap Singh Chundawat, Chandra Bandi
Keywords(s):
Category(s): Technology Classifications > Physical Sciences & Engineering, Technology Classifications > Healthcare & Life Sciences, Technology Classifications > Food, Nutrition and Nutraceuticals
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